原代地鼠肾细胞及狂犬病毒不同培养方式的研究

目的通过比较原代地鼠肾细胞在转瓶、微载体、细胞工厂的3种培养方式的培养效果,为原代地鼠肾细胞选择一种易扩大规模、培养高质量细胞的培养方式,进而提高狂犬病毒的产量。方法消化取得的细胞悬液分别在转瓶、微载体、细胞工厂中培养,通过显微镜观察细胞形态、计数等结果比对培养的差别。结果细胞工厂可以很好地培养原代地鼠肾细胞和狂犬病毒;而细胞在微载体上贴附性差,生长不好。结论实验结果表明细胞工厂可以取代转瓶,用于大规模培养原代地鼠肾细胞扩大狂犬病毒的产量。

Study of different culture modes for primary hamster kidney cells and rabies virus

Objective To select a culture mode that could be used in a large scale production for primary hamster kidney cells(PHKC) in high quality and then used the cells as a substrate to inhance production of rabies virus(RV),three culture modes,such as roller bottle,microcarrier and cell factory(CF) were taken and compered,respectively.Methods PHKC were digested and then cell suspensions were cultured in roller bottle,cell factory and on microcarrier,cell morpho-logy observed under microscope and cell number counted,accordingly.Results Rabies virus inoculated on PHKC was cultured very well in cell factory,but the ability of attachment of PHKC on microcarrier was poor and not cultured well.Conclusion In this study,it showed that cell factory can replace roller bottle to culture PHKC for cultivation rabies virus in a large scale.