| 无细胞百日咳菌苗毒性试验参考苗制备条件的探讨 |
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| 引用本文： | 王祖森,秦进才.无细胞百日咳菌苗毒性试验参考苗制备条件的探讨[J].微生物学免疫学进展,1998,26(1):13-18. |
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| 作者姓名： | 王祖森 秦进才 |
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| 作者单位： | 卫生部兰州生物制品研究所，中国药品生物制品检定所 |
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| 摘 要： | 将不同灭活条件下制备的无细胞百日咳毒性试验参考苗进行了检测。结果表明，以浓度０．１％Ｆｏｒｍａｌｉｎ溶液２５℃灭活９６－１２０小时制备的无细胞百日咳菌苗毒性试验参考苗，凝集效价仍可达到百日咳Ⅰ相血清原效价；不耐热毒素试验呈阴性；毒性试验ＢＷＤＵ／ｍｌ为７５．９－１２８．４；ＬＰＵ／ｍｌ为２．１－６．０；ＨＳＵ／ｍｌ为３．９－５．７；稳定性良好。该苗可标化作为无细胞百日咳菌苗毒性试验的参考标准
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| 关 键 词： | 无细胞百日咳菌苗毒性试验参考苗 小鼠体重减轻活性 小鼠白细胞增多促进活性 小鼠组胺致敏活性 |
Study on preparing condition of reference for toxicity test of acellular pertussis vaccine |
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| Abstract: | In this reports,the effect was detected in preparaing reference under the different formalin solution,temperature and inactivating time.The results indicated that the agglutination titer of prepared reference can reach the orginal level of pertussis I phase serum,especially,the preparation under 0.1% formalin at 25 ℃ for 96-120 hours.The freedom from heat labile toxicity test is negative.BWDU/ml is 75.9-128.4,LPU/ml is 2.1-6.0,HSU/ml is 3.9-5.7,stability is good.This vaccine can be used as reference in acellular pertussis vaccine toxicity test after standardization. |
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| Keywords: | Reference for acellular pertussis vaccine toxicity test Mouse body weight decreasing toxicity activity Mouse leukocytosis promoting activity Mouse histamine sensitizing activity |
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