Expression and Purification of a Functional Porcine Soluble Triggering Receptor Expressed on Myeloid Cells 1 |
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| Authors: | Hui Li Shuangshuang Guo Leyan Yan Chunhua Meng Yiyi Hu Kongwang He |
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| Institution: | 1. Key Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Science, Nanjing, China;2. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, China;3. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Science, Nanjing, China |
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| Abstract: | Triggering receptor expressed on myeloid cells 1 (TREM-1) plays a vital role in the pathogen-triggered amplification loop required for proinflammatory responses. Blockade of TREM-1 signaling may inhibit expansion of sepsis and prolong survival of animals. In the present study, the gene of porcine soluble TREM-1 was cloned and expressed in E. coli. After purification, the bioactivity of recombinant porcine soluble TREM-1 was tested in vitro on porcine alveolar macrophages. The results showed that supplementation with the recombinant porcine sTREM-1 protein rapidly and dose-dependently attenuated the upregulation of cytokines (IL-1β, IL-2, IL-4, IL-8, IL-10, IL-12, IL-16, IL-18, and TNF-α) caused by LPS stimulation in the cultured porcine alveolar macrophages. These results indicate that the recombinant porcine sTREM-1 protein can prevent TREM-1-mediated hyperinflammatory responses after exposure to LPS. |
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| Keywords: | Cytokines Inflammatory responses LPS Porcine TREM-1 |
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