Tetracycline‐controlled expression of glycosylated porcine interferon‐gamma in mammalian cells |
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Authors: | A Cencic F LeFèvre S Koren C La Bonnardière |
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Institution: | 1. Unité de Virologie et d'Immunologie Moléculaires , I.N.R.A. , Jouy‐en‐Josas, 78350, France;2. Faculty of Agriculture , University of Maribor , Vrbanska 30, Maribor, 2000, Slovenia;3. Unité de Virologie et d'Immunologie Moléculaires , I.N.R.A. , Jouy‐en‐Josas, 78350, France;4. Institute of Microbiology, Medical Faculty , University of Ljubljana , Zaloska 4, Ljubljana, 1000, Slovenia |
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Abstract: | Abstract Tetracycline‐controlled expression plasmids that allow inducible expression of proteins in mammalian cells (Gossen & Bujard, 1992), have been used to express porcine interferon‐γ in the RK‐13 rabbit kidney cell line. Following neomycin selection, stable clones produced recombinant, glycosylated porcine interferon‐γ (rGPoIFN‐γ) only after removal of tetracycline (Tc). Southern blot analysis of one clone showed that approximately 50 copies of IFN‐γ cDNA were present in the cell genome. In the absence of Tc, stable clones secreted large amounts of rGPoIFN‐γ (up to 16 μg/ml) into the medium supplemented with 10% FCS and high glucose concentration. Molecular weight comparison of 35S‐Methionine, labelled rGPoIFN‐γ with natural leukocytic IFN‐γ after immunoprecipitation, revealed 4 major glycoforms with apparent Mr of 27,000; 25,000; 20,000 and 18,500, that are almost identical in both IFN‐γ species. In both cases, all 4 glycoforms resolved into 2 polypeptide monomers with apparent Mr of 16,500 and 14,500 upon deglycosylation with N‐glycosydase F. The biological activity of rGPoIFN‐γ was in the same range as that of natural leukocytic PoIFN‐γ (2 × 106 U/mg). Eventually, this recombinant mammalian IFN‐γ should constitute a very useful substitute for leukocyte PoIFN‐γ in in vitro or in vivo experiments. |
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