Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products |
| |
| Authors: | Chris J C Johnston Elaine Robertson Yvonne Harcus John R Grainger Gillian Coakley Danielle J Smyth Henry J McSorley Rick Maizels |
| |
| Institution: | 1.Institute of Immunology and Infection Research, University of Edinburgh;2.Manchester Collaborative Centre for Inflammation Research |
| |
| Abstract: | Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES). |
| |
| Keywords: | Immunology Issue 98 Heligmosomoides polygyrus Helminth Life Cycle Excretory-Secretory Products Immunology Infection Mouse |
|
