Calcium Signaling in <Emphasis Type="Italic">Carassius</Emphasis> Cerebellar Neurons: Role of the Mitochondria |
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Authors: | I A Lukyanetz P G Kostyuk E A Lukyanetz |
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Institution: | (1) Institute for Limnology, Austrian Academy of Sciences, 5310 Mondseestrasse 9, A – Mondsee, Austria;(2) 2University of Lausanne (UNIL), Department of Ecology and Evolution (DEE) Biophore, 1015 Lausanne, CH, Switzerland |
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Abstract: | We studied the involvement of the mitochondria playing the role of a calcium store in the control of calcium exchange in cerebellar
neurons of a fish species tolerant to hypoxia, crucian (Carassius gibelio). In our experiments we used an ionophore, CCCP, that blocked accumulation of calcium by the above organelles. The intracellular
concentration of free Ca2+ (Ca2+]
і
) was measured using a calcium-sensitive dye, Fura-2AM, and the microfluorescent technique. We found that cerebellar neurons
of Carassius gibelio possess a well-expressed system clearing the cytoplasm from excessive Ca2+, and the mitochondria are actively involved in this process. Under conditions of suppression of the process of accumulation
of calcium by the mitochondria under the action of CCCP, the amplitude of calcium transients increased by about 50%. In addition,
the decay phase of depolarization-induced intracellular calcium transients was slowed down considerably. Therefore, our experiments
are indicative of the significant role of the mitochondria in the control of calcium dynamics in cerebellar neurons of Carassius gibelio in the course of functional activity of these cells. |
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