IL-13抑制剂在损伤大鼠尾椎间盘退变中的作用研究

为了探讨IL-13细胞因子在损伤后大鼠椎间盘退变中的影响，建立了大鼠尾椎间盘退变模型，给予IL-13抑制剂sIL-13Rα2-Fc进行干预，将实验分为空白、对照、低剂量、中剂量、高剂量干预组。分别于4周及6周后通过HE染色和Masson染色观察椎间盘形态变化并评分；DMMB法定量分析椎间盘中的糖胺多糖(glycosaminoglycan,GAG)、硫酸软骨素(chondroitin sulfate,CS)、硫酸角质素(keratan sulfate,KS)、透明质酸(hyaluronic acid,HA)含量变化；RT-PCR分析Ⅰ型和Ⅱ型胶原蛋白的mRNA表达水平；蛋白质印迹分析Ⅰ型和Ⅱ型胶原蛋白含量。HE和Masson染色显示与对照组相比，干预组椎间盘病理改变减小，纤维环排列更规则，破裂部位减小，NP细胞数量增加，胶原纤维减少。sIL-13Rα2-Fc干预增加了糖胺多糖、透明质酸含量，增加了硫酸软骨素/硫酸角质素比,减少了Ⅰ型胶原蛋白的表达,并增加了Ⅱ型胶原蛋白。结果表明IL-13抑制剂sIL-13Rα2-Fc可有效减轻椎间盘退变，并且与作用时间和浓度成正相关。

Research on the Role of IL-13 Inhibitors in Tail Disc Degeneration in Rats After Injury

To explore the effect of interleukin-13 signaling pathway on intervertebral disc degeneration in rats, we established a rat model of degeneration of the tail intervertebral disc. The experiment was divided into blank, control, low-dose, middle-dose, and high-dose intervention groups. The morphological changes of intervertebral discs by HE staining and Masson staining was observed and scored. Quantitative analysis of glycosaminoglycan, chondroitin sulfate, keratan sulfate, and hyaluronic acid content changes was analyzed in intervertebral discs by DMMB method. mRNA expression levels of typeⅠ and typeⅡ collagen was analyzed by RT-PCR analysis. TypeⅠand typeⅡ collagen content was analyzed by western blot analysis. HE and Masson staining results showed that the pathological changes of the intervertebral disc were reduced compared with the control group, the fibrous annulus arrangement was more regular, the rupture site was reduced, the number of NP cells was increased, and the collagen fibers were reduced. sIL-13Rα2-Fc intervention increased GAG and HA content, Ⅰ collagen and the CS/KS ratio, and decreased Ⅱ collagen content. These results indicated that sIL-13Rα2-Fc can effectively reduce the degeneration of the intervertebral disc, and it is positively correlated with the action time and concentration.