Molecular cloning of mannose-binding lectins from Clivia miniata |
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Authors: | Els J M Van Damme Koen Smeets Fred Van Leuven Willy J Peumans |
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Institution: | (1) Laboratory for Phytopathology and Plant Protection, Catholic University of Leuven, Willem de Croylaan 42, 3001 Leuven, Belgium;(2) Centrum Menselijke Erfelijkheid, Catholic University of Leuven, Herestraat 49, 3001 Leuven, Belgium |
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Abstract: | Screening of a cDNA library constructed from total RNA isolated from young developing ovaries of Clivia miniata Regel with the amaryllis lectin cDNA clone resulted in the isolation of four different isolectin clones which clearly differ from each other in their nucleotide sequences and hence also in their deduced amino acid sequences. Apparently the lectin is translated from an mRNA of ca. 800 nucleotides encoding a precursor polypeptide of 163 amino acids. Northern blot analysis of total RNA isolated from different tissues of Clivia miniata has shown that the lectin is expressed in most plant tissues with very high lectin mRNA concentrations in the ovary and the seed endosperm. |
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Keywords: | Clivia miniata cDNA cloning lectin |
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