Analysis of the genes of the OEE1 and OEE3 proteins of the photosystem II complex from Chlamydomonas reinhardtii |
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Authors: | Stephen P Mayfield Michèle Schirmer-Rahire Gerhard Frank Herbert Zuber Jean-David Rochaix |
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Institution: | (1) Department of Molecular Biology, University of Geneva, Geneva, Switzerland;(2) Department of Plant Biology, University of Geneva, Geneva, Switzerland;(3) Eidgenössische Technische Hochschule, Hönggerberg, CH-8093 Zürich, Switzerland;(4) Present address: Department of Molecular Biology, Research Institute of Scripps Clinic, 10666 North Torrey Pines Rd, 92037 La Jolla, CA, USA |
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Abstract: | The sequences of the nuclear genes of the 33 kDa (OEE1) and the 16 kDa (OEE3) polypeptides of the oxygen evolving complex of Chlamydomonas reinhardtii have been established. Comparison between the OEE1 protein sequences of C. reinhardtii and higher plants and cyanobacteria reveals 67 and 47% homology. In contrast, C. reinhardtii and higher plants have only 28% overall homology for OEE3 which is mostly limited to the central portion of the protein. The transit peptides of the C. reinhardtii proteins consist of 52 (OEE1) and, most likely, 51 (OEE1) amino acids. They have a basic amino terminal region and, at least in the case of OEE1, a hydrophobic segment at their carboxy terminal end typical of thylakoid lumen proteins. Comparison of the genomic and cDNA clones indicates that the OEE1 and OEE3 genes contain five and four introns, respectively, some of which are located within the coding sequences of the transit peptides. |
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Keywords: | Chlamydomonas reinhardtii chloroplast oxygen evolving complex photosystem II transit peptides |
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