Small-scale field tests with transgenic potato,cv. Bintje,to test resistance to primary and secondary infections with potato virus y |
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Authors: | Pia Malnoë Laurent Farinelli Gerald F Collet Werner Reust |
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Institution: | (1) Present address: Federal Agricultural Research Station of Changins (RAC), CH-1260 Nyon, Switzerland;(2) Department of Plant Biology, University of Geneva, CH-1205 Geneva, Switzerland;(3) Departemento de Genetica-Instituto de Biologia, Universidade Federal do Rio de Janeiro, UFRJ, CP 68011, 21941 Rio de Janeiro, Brasil |
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Abstract: | The coat protein (CP) gene of the potato virus Y (PVY) strain N605 has been cloned into a plant binary expression vector and introduced into the potato variety Bintje. The transformed lines, Bt6, that contained two copies of the CP gene showed complete resistance to the homologous strain PVY-N605 and a good resistance to the related strain PVY-O803 in the greenhouse. The good resistance of Bt6 to primary and secondary infections by PVY was confirmed in two successive field tests where the virus was transmitted by its natural aphid vector. |
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Keywords: | coat protein-mediated resistance field test plant virus potyvirus transgenic potato |
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