The plant mitochondrial open reading frame orf221 encodes a membrane-bound protein |
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Authors: | Laudenir M Prioli Jintai Huang Charles S Levings III |
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Institution: | (1) Departamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Cx. Postal 6109, CEP13081 Campinas, SP, Brasil;(2) Department of Genetics, North Carolina State University, Box 7614, 27695-7614 Raleigh, NC, USA |
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Abstract: | We have shown that the open reading frame orf221 is an active mitochondrial gene which encodes a novel mitochondrial polypeptide. The orf221 sequence is common to higher plants but absent in animal and fungal mitochondria. A mitochondrial polypeptide with an apparent molecular weight of 21 000 was detected with a polyclonal antibody raised against an ORF221 fusion protein. In organello translation followed by immunoprecipitation with the anti-ORF221 antibody demonstrated that this polypeptide is encoded by the orf221 gene in plant mitochondria. The ORF221 was found to be a mitochondrial membrane protein in normal (N), cms-T, and cms-C cytoplasms of several inbred lines of maize (Zea mays L.) and in other plant species. |
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Keywords: | mitochondrial DNA mitochondrial gene expression mitochondrial protein ORF25 T-urf13 |
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