Transgenic <Emphasis Type="Italic">Arabidopsis</Emphasis> and tobacco plants overexpressing an aquaporin respond differently to various abiotic stresses |
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Authors: | Ji Young Jang Seong Hee Lee Ji Ye Rhee Gap Chae Chung Sung Ju Ahn Hunseung Kang |
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Institution: | (1) Department of Plant Biotechnology, Agricultural Plant Stress Research Center and Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Korea |
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Abstract: | Despite the high isoform multiplicity of aquaporins in plants, with 35 homologues including 13 plasma membrane intrinsic proteins
(PIPs) in Arabidosis thaliana, the individual and integrated functions of aquaporins under various physiological conditions remain unclear. To better understand
aquaporin functions in plants under various stress conditions, we examined transgenic Arabidopsis and tobacco plants that constitutively overexpress Arabidopsis PIP1;4 or PIP2;5 under various abiotic stress conditions. No significant differences in growth rates and water transport
were found between the transgenic and wild-type plants when grown under favorable growth conditions. The transgenic plants
overexpressing PIP1;4 or PIP2;5 displayed a rapid water loss under dehydration stress, which resulted in retarded germination
and seedling growth under drought stress. In contrast, the transgenic plants overexpressing PIP1;4 or PIP2;5 showed enhanced
water flow and facilitated germination under cold stress. The expression of several PIPs was noticeably affected by the overexpression of PIP1;4 or PIP2;5 in Arabidopsis under dehydration stress, suggesting that the expression of one aquaporin isoform influences the expression levels of other
aquaporins under stress conditions. Taken together, our results demonstrate that overexpression of an aquaporin affects the
expression of endogenous aquaporin genes and thereby impacts on seed germination, seedling growth, and stress responses of
the plants under various stress conditions.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Abiotic stress Aquaporin Arabidopsis thaliana Tobacco Transgenic plants Water channel |
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