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Expression of a synthetic antifreeze protein in potato reduces electrolyte release at freezing temperatures
Authors:Wallis  James G  Wang  Hongyu  Guerra  Daniel J
Institution:(1) Present address: Institute of Biological Chemistry, Washington State University, Pullman, WA;(2) Present address: Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey, USA;(3) Department of Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844, USA
Abstract:A synthetic antifreeze protein gene was expressed in plants and reduced electrolyte leakage from the leaves at freezing temperatures. The synthetic AFP was expressed as a fusion to a signal peptide, directing it to the extracytoplasmic space where ice crystallization first occurs. The gene was introduced to Solanum tuberosum L. cv. Russet Burbank by Agrobacterium-mediated transformation. Transformants were identified by PCR screening and expression of the introduced protein was verified by immunoblot. Electrolyte-release analysis of transgenic plant leaves established a correlation between the level of transgenic protein expression and degree of tolerance to freezing. This is the first identification of a phenotype associated with antifreeze protein expression in plant tissue.
Keywords:transgenic Russet Burbank  cold tolerance
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