Chemical- and Irradiation-induced Mutants of Indica Rice IR64 for Forward and Reverse Genetics |
| |
Authors: | Jian-Li Wu Chanjian Wu Cailin Lei Marietta Baraoidan Alicia Bordeos Ma Reina Suzette Madamba Marilou Ramos-Pamplona Ramil Mauleon Arlett Portugal Victor Jun Ulat Richard Bruskiewich Guoliang Wang Jan Leach Gurdev Khush Hei Leung |
| |
Institution: | (1) Entomology and Plant Pathology Division, International Rice Research Institute, DAPO 7777, Metro Manila, Philippines;(2) National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006, China;(3) Biometrics and Bioinformatics Unit, International Rice Research Institute, DAPO 7777, Metro Manila, Philippines;(4) Department of Plant Pathology, Ohio State University, Columbus, OH 43210, USA;(5) Department of Plant Pathology, Kansas State University, Manhattan, KS 66506-5502, USA |
| |
Abstract: | IR64, the most widely grown indicarice in South and Southeast Asia, possesses many positive agronomic characteristics (e.g., wide adaptability, high yield potential,
tolerance to multiple diseases and pests, and good eating quality,) that make it an ideal genotype for identifying mutational
changes in traits of agronomic importance. We have produced a large collection of chemical and irradiation-induced IR64 mutants
with different genetic lesions that are amenable to both forward and reverse genetics. About 60,000 IR64 mutants have been
generated by mutagenesis using chemicals (diepoxybutane and ethylmethanesulfonate) and irradiation (fast neutron and gamma
ray). More than 38,000 independent lines have been advanced to M4 generation enabling evaluation of quantitative traits by replicated trials. Morphological variations at vegetative and reproductive
stages, including plant architecture, growth habit, pigmentation and various physiological characters, are commonly observed
in the four mutagenized populations. Conditional mutants such as gain or loss of resistance to blast, bacterial blight, and
tungro disease have been identified at frequencies ranging from 0.01% to 0.1%. Results from pilot experiments indicate that
the mutant collections are suitable for reverse genetics through PCR-detection of deletions and TILLING. Furthermore, deletions
can be detected using oligomer chips suggesting a general technique to pinpoint deletions when genome-wide oligomer chips
are broadly available. M4 mutant seeds are available for users for screening of altered response to multiple stresses. So far, more than 15,000 mutant
lines have been distributed. To facilitate broad usage of the mutants, a mutant database has been constructed in the International
Rice Information System (IRIS; http: //www.iris.irri.org) to document the phenotypes and gene function discovered by users. |
| |
Keywords: | functional genomics mutagenesis Oryza sativa phenotype database |
本文献已被 PubMed SpringerLink 等数据库收录! |
|