首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Structural organization and differential expression of three stilbene synthase genes located on a 13 kb grapevine DNA fragment
Authors:Wilfried Wiese  Barbara Vornam  Elvira Krause  Helmut Kindl
Institution:(1) Biochemie, Fachbereich Chemie, Philipps-Universität, Hans-Meerwein-Strasse, D-35032 Marburg, FRG;(2) Bayer AG, Institut für Biotechnologie, D-51368 Leverkusen, FRG
Abstract:A 13 kb DNA fragment was isolated from a grapevine (Vitis var. Optima) genomic library by hybridizing with elicitor-induced stilbene synthase cDNA as a probe. After fragmentation with Eco RI, subcloning and sequencing, two full-size stilbene synthase genes (Vst1 and Vst2) and the 3prime end of a third stilbene synthase gene (Vst3) were located within the 13 kb fragment. Vst1 and Vst2, differing only slightly in the coding region, are distinguished in the intron size and in the structure of the promoter region. The 5prime flanking region of gene Vst1 contains a TATAA box at nucleotide –48. The substantial structural differences found for the promoters of the two genes are paralleled by a striking difference in the expression of the two genes in elicitor-treated cells. Moreover, the accumulation upon elicitation of six different stilbene synthase mRNAs was studied and found to differ by two orders of magnitude.
Keywords:gene sequences  grapevine  induction by elicitor  phytoalexin formation  promoter  stilbene synthase  Vitis
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号