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Transposon tagging in rice |
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| Authors: | Izawa Takeshi Ohnishi Tohru Nakano Toshitsugu Ishida Nobuhiro Enoki Hiroyuki Hashimoto Hisako Itoh Kimiko Terada Rie Wu Chuanyn Miyazaki Chikara Endo Tomoko Iida Shigeru Shimamoto Ko |
| Institution: | (1) Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, 630-01, Japan;(2) Plantech Research Institute, 1000 Kamoshida, Aoba-ku, Yokohama, 227, Japan;(3) Department of Biological Science and Technology, Science University of Tokyo, Yamazaki, Noda, 278, Japan;(4) Present address: National Institute for Basic Biology, 38 Nishigonaka Myodaijicho, Okazaki, 444, Japan;(5) Present address: Department of Agriculture, Niigata University, 8050 Igarashi, Ninomachi, Niigata, 950-21, Japan;(6) Present address: National Institute of Agrobiological Resources, 212 Kannondai, Tsukuba, 305, Japan;(7) Present address: Nagasaki Laboratory, Japan Science and Technology Cooperation, 2-1303 Ikeda, Ohmura, 856, Japan;(8) Present address: National Institute of Fruit Tree Science, Okitsu, Shimizu, 424-02, Japan |
| Abstract: | To develop an efficient gene isolation method for rice we introduced the maize Ac/Ds system into rice. Extensive analysis of their behavior in rice for several generations indicated that Ac and Ds in the presence of Ac transposase gene actively transpose in rice. A wide spectrum of mutations affecting growth, morphogenesis, flowering time and disease resistance have been obtained in the population carrying Ac/Ds and some of them were genetically analyzed. Main efforts are currently being made to isolate genes responsible these mutations. In addition, a number of Ac/Ds were mapped on chromosomes and mapped elements will be used in the future for directed tagging of genes with known chromosomal positions. |
| Keywords: | rice Ac/Ds transposon gene isolation disease resistance flowering time |
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