Successful expression in pollen of various plant species of in vitro synthesized mRNA introduced by particle bombardment |
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| Authors: | Toshinori Tanaka Masahiro Nishihara Motoaki Seki Atsushi Sakamoto Kunisuke Tanaka Kohei Irifune Hiromichi Morikawa |
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| Institution: | (1) Graduate Department of Gene Science, Faculty of Science, Hiroshima University, 739 Higashi-Hiroshima, Japan;(2) Department of Biochemistry, College of Agriculture, Kyoto Prefectural University, 606 Kyoto, Japan;(3) Present address: National Institute for Basic Biology, Myodaiji, 444 Okazaki, Japan |
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| Abstract: | Gold particles coated with  -glucuronidase (GUS) mRNA with a 5 cap structure that had been synthesized in vitro were introduced, by use of a pneumatic particle gun, into pollen grains of lily (Lilium longiflorum), freesia (Freesia refracta) and tulip (Tulipa gesneriana). A fluorometric assay for the GUS activity indicated that in vitro synthesized GUS mRNA introduced into these pollen cells by particle bombardment was successfully expressed. GUS activity in extracts of the bombarded lily pollen became detectable fluorometrically within 30 min after bombardment, peaked at 6 h, then gradually decreased. This activity changed as a function of the developmental stage of the pollen cell of lily. |
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| Keywords: | Lilium longiflorum Freesia refracta Tulipa gesneriana GUS mRNA particle bombardment pollen |
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