Expression of the Hypersensitive Response-assisting Protein
in <Emphasis Type="Italic">Arabidopsis</Emphasis> Results in Harpin-dependent Hypersensitive
Cell Death in Response to <Emphasis Type="Italic">Erwinia carotovora</Emphasis> |
| |
Authors: | Ajay-Kumar?Pandey Mang-Jye?Ger Hsiang-En?Huang Mei-Kuen?Yip Jiqing?Zeng Email author" target="_blank">Teng-Yung?FengEmail author |
| |
Institution: | (1) Institute of Plant and Microbial Biology, Academia Sinica 115, Taipei, Taiwan;(2) Department of Life Science, National University of Kaohsiung, 811 Kaohsiung, Taiwan |
| |
Abstract: | Active defense mechanisms of plants against pathogens often include a rapid plant cell death known as the hypersensitive cell
death (HCD). Hypersensitive response-assisting protein (HRAP) isolated from sweet pepper intensifies the harpinPss-mediated HCD. Here we demonstrate that constitutive expression of the hrap gene in Arabidopsis results in an enhanced disease resistance towards soft rot pathogen, E. carotovora subsp. carotovora. This resistance was due to the induction of HCD since different HCD markers viz. Athsr3, Athsr4, ion leakage, H2O2 and protein kinase were induced. One of the elicitor harpin proteins, HrpN, from Erwinia carotovora subsp. carotovora was able to induce a stronger HCD in hrap-Arabidopsis than non-transgenic controls. To elucidate the role of HrpN, we used E. carotovora subsp. carotovora defective in HrpN production. The hrpN− mutant did not induce disease resistance or HCD markers in hrap-Arabidopsis. These results imply that the disease resistance of hrap-Arabidopsis against a virulent pathogen is harpin dependent. |
| |
Keywords: | Arabidopsis thaliana Erwinia carotovora harpin HRAP hypersensitive response |
本文献已被 PubMed SpringerLink 等数据库收录! |
|