Molecular analysis of early rice stamen development
using organ-specific gene expression profiling |
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Authors: | Xiao-Chun Lu Hua-Qin Gong Mo-Li Huang Su-Lan Bai Yang-Bo He Xizeng Mao Zhi Geng Song-Gang Li Liping Wei Jie-Shuai Yuwen Zhi-Hong Xu Shu-Nong Bai |
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Institution: | (1) PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, Beijing, 100871, China;(2) Department of Probability and Statistics, School of Mathematical Sciences, Peking University, Beijing, 100871, China;(3) National Plant Gene Research Center (Beijing), Beijing, 100871, China |
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Abstract: | Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and
will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial
cells differentiates into an organ, through a well-orchestrated series of events, with a given shape, structure and function.
Research over the past two decades has elucidated the molecular mechanisms of organ identity and dorsalventral axis determinations.
However, little is known about the molecular mechanisms underlying the successive processes. To develop an effective approach
for studying organ formation at the molecular level, we generated organ-specific gene expression profiles (GEPs) reflecting
early development in rice stamen. In this study, we demonstrated that the GEPs are highly correlated with early stamen development,
suggesting that this analysis is useful for dissecting stamen development regulation. Based on the molecular and morphological
correlation, we found that over 26 genes, that were preferentially up-regulated during early stamen development, may participate
in stamen development regulation. In addition, we found that differentially expressed genes during early stamen development
are clustered into two clades, suggesting that stamen development may comprise of two distinct phases of pattern formation
and cellular differentiation. Moreover, the organ-specific quantitative changes in gene expression levels may play a critical
role for regulating plant organ formation.
Electronic Supplementary Material Supplementary material is available for this article at
Xiao-Chun Lu, Hua-Qin Gong contributed equally to this work. |
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Keywords: | Rice Organ formation Early stamen development Organ-specific gene expression profiling Microarray analysis |
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