Molecular characterization of salt-stress-associated protein in citrus: protein and cDNA sequence homology to mammalian glutathione peroxidases |
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Authors: | Doron Holland Gozal Ben-Hayyim Zehava Faltin Luc Camoin A Donny Strosberg Yuval Eshdat |
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Institution: | (1) Department of Fruit Tree Breeding and Genetics, Agricultural Research Organization, The Volcani Center, 50250 Bet Dagan, Israel;(2) Laboratoire d'Immuno-Pharmacologie Moléculaire, CNRS, Université Paris VII, 22 Rue Méchain, 75014 Paris, France;(3) Institut Cochin de Génetique Moléculaire, 22 Rue Méchain, 75014 Paris, France |
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Abstract: | A gene encoding for a citrus salt-stress-associated protein (Cit-SAP) was cloned from Citrus sinensis salt-treated cell suspension. The gene, designated csa, was isolated from a cDNA expression library. The partial amino acid sequence of the protein, as well as that deduced from the nucleotide sequence of csa, revealed a considerable homology to mammalian glutathione peroxidase (GP), and to clone 6P229 from tobacco protoplasts. The increased expression of Cit-SAP in NaCl-treated cultured citrus cells and in citrus plants irrigated with saline water, and its similarity to GP, raise the possibility that one of the effects of salt stress in plants may be the increase of the level of free radicals. |
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Keywords: | Citrus sinensis gene isolation glutathione peroxidase salt stress protein sequence |
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