Sequencing and characterization of the kinesin-related geneskatB andkatC ofArabidopsis thaliana |
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| Authors: | Hisayuki Mitsui Kimihiko Nakatani Kazuko Yamaguchi-Shinozaki Kazuo Shinozaki Ken Nishikawa Hideo Takahashi |
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| Institution: | (1) Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan;(2) Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Tsukuba, Japan;(3) Protein Engineering Research Institute, Suita, Osaka, Japan |
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| Abstract: | Complementary DNAs of two kinesin-related genes,katB andkatC, were isolated fromArabidopsis thaliana and sequenced. The carboxyl-terminal regions of the polypeptides encoded by these genes, especially the presumptive ATP-binding and microtubule-binding domains, share significant sequence homology with the mechanochemical motor domain of the kinesin heavy chain. The predicted secondary structures of KatB and KatC proteins include a large globular domain in the carboxyl-terminal region and a small globular domain in the amino-terminal region that are separated by a long  -helical coiled-coil with heptad repeats. A truncated KatC polypeptide (KatC(207–754)), which includes the carboxylterminal region of KatC, was expressed inEscherichia coli and was shown to possess microtubule-stimulated ATPase activity and to bind to microtubules in an ATP-sensitive manner, both of which are characteristics of kinesin and kinesin-like proteins. |
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| Keywords: | Arabidopsis thaliana nucleotide sequences kat genes kinesin-like proteins microtubule-stimulated ATPase |
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