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A complete sequence of the rice sucrose synthase-1 (RSs1) gene
Authors:Ming-Bo Wang  Donald Boulter  John A. Gatehouse
Affiliation:(1) Department of Biological Sciences, University of Durham, South Road, DH1 3LE Durham, UK
Abstract:Using a fragment of the maize sucrose synthase gene Sh-1 as probe, the rice genome was shown to contain at least three genes encoding sucrose synthase. One of these genes was isolated from a genomic library, and its full sequence, including 1.7 kb of 5prime flanking sequence and 0.9 kb of 3prime flanking sequence, is reported. The new rice gene, designated RSs1, is highly homologous to maize Sh-1 (approx. 94% identity in derived amino acid sequence), and contains an identical intron-exon structure (16 exons and 15 introns). Both RSs1 and maize Sh-1 show similar sequence homologies to a second rice sucrose synthase gene described recently (designated RSs2, Yu et al. (1992) Plant Mol Biol 18: 139–142), although both the rice genes predict an extra 6 amino acids at the C-terminus of the protein when compared to the maize gene. The RSs1 5prime flanking sequence contains a number of promoter-like sequences, including putative protein-binding regions similar to maize zein genes.
Keywords:rice (Oryza sativa)  sucrose synthase  gene  DNA sequence
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