A complete sequence of the rice sucrose synthase-1 (RSs1) gene |
| |
Authors: | Ming-Bo Wang Donald Boulter John A. Gatehouse |
| |
Affiliation: | (1) Department of Biological Sciences, University of Durham, South Road, DH1 3LE Durham, UK |
| |
Abstract: | Using a fragment of the maize sucrose synthase gene Sh-1 as probe, the rice genome was shown to contain at least three genes encoding sucrose synthase. One of these genes was isolated from a genomic library, and its full sequence, including 1.7 kb of 5 flanking sequence and 0.9 kb of 3 flanking sequence, is reported. The new rice gene, designated RSs1, is highly homologous to maize Sh-1 (approx. 94% identity in derived amino acid sequence), and contains an identical intron-exon structure (16 exons and 15 introns). Both RSs1 and maize Sh-1 show similar sequence homologies to a second rice sucrose synthase gene described recently (designated RSs2, Yu et al. (1992) Plant Mol Biol 18: 139–142), although both the rice genes predict an extra 6 amino acids at the C-terminus of the protein when compared to the maize gene. The RSs1 5 flanking sequence contains a number of promoter-like sequences, including putative protein-binding regions similar to maize zein genes. |
| |
Keywords: | rice (Oryza sativa) sucrose synthase gene DNA sequence |
本文献已被 SpringerLink 等数据库收录! |
|