Effect of chloramphenicol and lincomycin on chloroplast DNA amplification in greening pea leaves |
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| Authors: | Yukiko Sasaki Tsuneyoshi Kuroiwa |
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| Institution: | (1) Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, 606 Kyoto, Japan;(2) Department of Botany, Faculty of Science, University of Tokyo, 113 Tokyo, Japan |
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| Abstract: | The light-induced increase in chloroplast DNA was not inhibited by two inhibitors of protein synthesis on 70S polysomes, chloramphenicol and lincomycin, in greening pea leaves. The changes in chloroplast DNA were observed by fluorescence microscopy and measured by hybridization to specific cloned probes. The results suggest that the light-induced increase in chloroplast DNA proceeds without de novo protein synthesis in the chloroplast, in agreement with those with mutants and cultured leaf tissue. |
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| Keywords: | chloramphenicol chloroplast DNA amplification fluorescence microscopy light induction lincomycin pea leaves |
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