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Structure of a functional geranylgeranyl pyrophosphate synthase gene from Capsicum annuum
Authors:Alfredo Badillo  Johannes Steppuhn  Jean Deruère  Bilal Camara  Marcel Kuntz
Institution:(1) Institut de Biologie Moléculaire des Plantes du CNRS, Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France;(2) Université Louis Pasteur, Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France
Abstract:A geranylgeranyl pyrophosphate synthase (GGPPS) gene from Capsicum annuum (bell pepper) was cloned. The nucleotide sequence shows that this gene, like the capsanthin/capsorubin gene but unlike the phytoene synthase gene from C. annuum, is not interrupted by an intron. Southern blot analysis of C. annuum genomic DNA suggests the presence of a single gene highly similar to the cDNA and also of additional related sequences. The present data suggest that this cloned gene is functional.
Keywords:bell pepper  carotenoid  chromoplast  fruit ripening
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