A promoter directing high level expression in pistils of transgenic plants |
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Authors: | Ficker Michael Wemmer Thomas Thompson Richard D |
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Institution: | (1) Present address: MRC Mammalian Genetics Unit, Harwell, Didcot, Oxfordshire, OX11 ORD, UK;(2) Institute of Neurophysiology, University of Duesseldorf, PO Box 101007, 40001 Duesseldorf, Germany;(3) Max Planck Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Cologne, Germany |
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Abstract: | The promoter of the potato (Solanum tuberosum L.) SK2 gene, encoding a pistil-specific basic endochitinase, was cloned. Various fragments of the SK2-promoter, from 1 kb down to 0.23 kb in length, were fused to the GUS reporter gene. Chimaeric SK2 promoter-GUS fusion constructs were transformed into potato by Agrobacterium tumefaciens-mediated transformation. The SK2-GUS transgenic potato plants exhibited a highly specific GUS activity in the pistil. Expression in the pistil was shown to be developmentally regulated. In addition to the GUS activity in pistils, transgenic plants also showed a much weaker ectopic expression in anthers. In other tissues no systematic expression was detectable. All SK2 promoter fragments analysed conferred pistil-specific expression without significant qualitative or quantitative differences, demonstrating that the regulatory elements mediating this expression pattern are located within a 230 bp SK2 promoter fragment. The SK2 promoter may be used to engineer high levels of expression in pistils of transgenic plants. |
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Keywords: | endochitinase pollination style transmitting tract |
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