Molecular cloning of a chloroplastic proteinassociated with both the envelope and thylakoid membranes |
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| Authors: | Hsou-min Li Yasuko Kaneko Kenneth Keegstra |
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| Institution: | (1) Department of Botany, University of Wisconsin, 53706 Madison, WI, USA;(2) Present address: Plant Biology Laboratory, The Salk Institute, P.O. Box 85800, 92186-5800 San Diego, CA, USA;(3) Present address: Department of Regulation Biology, Faculty of Science, Saitama University, 338 Urawa, Japan;(4) Present address: MSU-DOE Plant Research Laboratory, Michigan State University, 48824-1312 East Lansing, MI, USA |
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| Abstract: | Chloroplasts consist of six morphologically distinct compartments. Each compartment has a specific set of polypeptides that perform distinct biochemical functions. We report here the identification of a membrane-associated protein with a novel localization. This protein was synthesized as a 37 kDa precursor and was processed to a mature protein of 30 kDa after being imported into isolated pea chloroplasts. Fractionation of chloroplasts showed that the 30 kDa mature protein was associated with both of the envelope membranes as well as with thylakoid membranes. Immunocyto-chemical localization of the 30 kDa protein revealed that the protein occurred in clusters in the vicinity of both the envelope and the thylakoid. Possible functions of this 30 kDa protein, inferred from its novel localization pattern, are discussed.Abbreviations CAB
light-harvesting chlorophyll a/b-binding protein of photosystem II
- prCAB
precursor protein to CAB
- SS
small subunit of ribulose-1,5-bisphosphate carboxylase
- prSS
precursor protein to SS
- RCF
relative centrifugation force |
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| Keywords: | chloroplasts envelope localization precursor protein thylakoid |
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