Organellar DNA replication inNicotiana tabacum cultured cells |
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Authors: | Diógenes H Infante Arthur Weissbach |
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Institution: | (1) Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Roche Research Center, 07110 Nutley, NJ, USA;(2) Present address: Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, Box 244, 10021 New York, NY, USA |
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Abstract: | In the diploid vegetative plant cell, the nuclear DNA is present in two copies, whereas the chloroplast and mitochondria genomes are present in a higher and variable copy number. We have studied the replication of the nuclear, chloroplast and mitochondrial DNA in culturedNicotiana tabacum cells using density and radioactive markers. Essentially all the 10 000 chloroplast genomes in a given cell replicate in one cell cycle as do all the mitochondrial DNA molecules. No measurable level of unreplicated organellar DNA molecules can be detected in these cells. |
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Keywords: | BudR chloroplast DNA replication mitochondria nuclear |
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