实时定量PCR芯片——功能基因组研究的有用工具 |
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引用本文: | 曲佳,;熊鸣,;赵晓航.实时定量PCR芯片——功能基因组研究的有用工具[J].生命科学,2009(2):241-245. |
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作者姓名: | 曲佳 ;熊鸣 ;赵晓航 |
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作者单位: | [1]军事医学科学院,北京100850; [2]海军总医院,北京100037; [3]中国医学科学院肿瘤研究所,北京100021 |
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基金项目: | 总后医药卫生科研计划(06MB022);海后司令部科研计划(07-3309):“973”项目(2004CB518707) |
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摘 要: | 结合实时定量PCR(real-time quantitative polymerase chain reaction,qPCR)和基因芯片技术,实时定量PCR芯片(qPCR array)为功能基因组研究提供了一种高度灵敏、可靠的方法。在基因功能分类基础上,结合qPCR准确定量的特性,qPCR array可同时定量分析上百个基因的mRNA表达水平,是研究一组特定功能基因表达的理想方法。
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关 键 词: | 实时定量PCR芯片 信号通路分析 基因表达 |
Quantitative PCR array: a useful tool for functional genomic studies |
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Institution: | QU Jia, XIONG Ming, ZHAO Xiao-hang. (1 Academy of Military Medical Sciences, Beijing 100850, China; 2 Navy General Hospital, Beijing 100037, China; 3 Cancer Institute & Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China) |
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Abstract: | Combined with two techniques of real-time quantitative polymerase chain reaction (qPCR) and gene chips, the qPCR array brings a highly sensitive and reliable method of gene expression profiling. It allows quantitatively monitoring expression levels of panel genes in a specific pathway or disease related genes simultaneously, and is a useful tool for functional geneomic studies recently. |
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Keywords: | qPCR array signal transduction analysis gene expression |
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