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| 一种利用植物病毒载体表达外源蛋白的新平台——Magnifection | |
| 引用本文： | 杨正安,丁玉梅,张应华,刘飞虎.一种利用植物病毒载体表达外源蛋白的新平台——Magnifection[J].生命科学,2007,19(4):456-460. |
| 作者姓名： | 杨正安 丁玉梅 张应华 刘飞虎 |
| 作者单位： | 1. 云南农业大学园林园艺学院,昆明,650201;云南大学生命科学学院,昆明,650091 2. 云南省农业生物技术重点实验室,昆明,650223 3. 云南农业大学园林园艺学院,昆明,650201 4. 云南大学生命科学学院,昆明,650091 |
| 基金项目： | 国家自然科学基金;云南省自然科学基金 |
| 摘 要： | 本文介绍国内外在利用植物病毒表达载体生产药物蛋白的研究现状,并对这一领域取得的最新突破进行重点阐述,包括Magnifection的原理、技术流程及利用其生产重组药用蛋白的优势、存在的问题等.最后,结合相关经验介绍利用植物病毒表达载体生产药物蛋白的应用前景及对该技术改进的建议. |
| 关 键 词： | 植物 病毒载体 瞬时表达 |
| 文章编号： | 1004-0374（2007）04-0456-05 |
| 收稿时间： | 2006-12-23 |
| 修稿时间： | 2007-04-02 |
Magnifection: a new platform for expression of foreign protein using a plant viral-based vector |
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| YANG Zhengan,DING Yumei,ZHANG Yinghua,LIU Feihu.Magnifection: a new platform for expression of foreign protein using a plant viral-based vector[J].Chinese Bulletin of Life Sciences,2007,19(4):456-460. | |
| Authors: | YANG Zhengan DING Yumei ZHANG Yinghua LIU Feihu |
| Institution: | 1 College of Horticulture and Landscape, Yunnan Agricultural University, Kunming 650201, China; 2 Key Lab of Agricultural Biotechnology of Yunnan, Kunming 650223, China; 3 School of Life Sciences, Yunnan University, Kunming 650091, China |
| Abstract: | This article reviews the current research status of expression of recombinant proteins using plant viralbased vector, and more attentions are focused on the principle and the operation procedure of Magnifection and its advantages and limitation for the production of recombinant pharmaceutical proteins, lastly, we introduce its application in diverse aspects of understanding the mechanisms of RNA processing and nuclear export in plant, gene replacement, gene insertion, epitope presentation, gene complementation and commercial purpose. Our experiences in modification of the Magnifection technique are also provided. |
| Keywords: | Magnifection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |