低温电子显微技术在膜蛋白结构研究中的应用和展望

介绍了蛋白质电子晶体学和单颗粒分析技术这两种低温电子显微技术在膜蛋白和膜蛋白复合体结构研究中的具体方法和近10~20年来的实际应用,并分别分析了这两种方法的优势和瓶颈。此外,还介绍了Amphipol替代、Streptavidin二维晶体锚定脂质体和纳米球包被脂质体等近两年来出现的新的用于低温电镜成像的膜蛋白样品制备方法。最后对膜蛋白的低温电子显微研究的未来发展做了展望。

Application and aspect of cryo-electron microscopy for the structural study of membrane proteins

Two three-dimensional cryo-electron microscopic techniques,electron crystallography and single particle analysis,and their application for the structural study of membrane proteins and membrane complexes in the recent ten to twenty years were introduced and reviewed.The benefits and drawbacks of those two methods on the membrane protein structural study were discussed accordingly.Besides,the recent appeared new sample preparation techniques for cryo-electron microscopic study of membrane proteins,e.g.the amphipol usage,liposome tethered by 2D crystal of streptavidin and solid-based liposome,were also introduced and discussed.Finally,I gave my personal aspect of the future development of cryo-electron microscopy on its potential of solving the 3D structure of membrane proteins.