| 几种差别表达基因显示技术及其在植物方面的应用 |
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| 引用本文： | 宁顺斌,王玲.几种差别表达基因显示技术及其在植物方面的应用[J].生命科学,1999,11(3):140-143,144. |
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| 作者姓名： | 宁顺斌 王玲 |
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| 作者单位： | 武汉大学发育生物学研究中心!武汉４３００７２ |
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| 摘 要： | ９０年代以来，先后出现了ＤＤ，ＲＤＡ，ｃＤＮＡ－ＲＤＡ，ＳＡＧＥ，ＧＥＦ，ＲＦＤ，ＳＳＨ以及ＡＴＡＣ－ＰＣＲ等数种未知产物的差别表达基因显示技术，本文对这些技术的异同。各自的优缺点以及它们在植物方面的应用现状和前景作了简单综述。
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| 关 键 词： | RT－PCR 差别表达基因 基因克隆 |
A set of techniques for differentially-expressed-gene display and their applications in plants |
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| NING Shun-Bin, WANG Ling, SONG Yun-Chun,.A set of techniques for differentially-expressed-gene display and their applications in plants[J].Chinese Bulletin of Life Sciences,1999,11(3):140-143,144. |
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| Authors: | NING Shun-Bin WANG Ling SONG Yun-Chun |
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| Abstract: | in the resent ten years,a set of techniques for display of differentially expressedgene have been established, which include DD (differential displayl, RDA (representational dif-ference analysis), cDNA-RDA,SAGE(serial analysis of gene expression), GEF (gene expressionfingerprintingi,RFD(display of cDNA 3'-end-restriction fragments), SSH (suppression subtrac-tive hybridization) and ATAC-PCR (edaptortagged competitive PCR).In the present paper,thecommon and different aspects among these techniques and the advantages and defects of each werediscussed as well as their applications and prospects in plants. |
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| Keywords: | RT-PCR differentially expressed gene gene cloning adaptor false positive |
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