A combined pathway of somatic embryogenesis and organogenesis to regenerate radiata pine plants |
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Authors: | I A Montalbán N De Diego E Aguirre Igartua A Setién P Moncaleán |
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Institution: | (1) Neiker Tecnalia, Centro de Arkaute, 01080 Vitoria-Gasteiz, Spain; |
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Abstract: | This study describes for the first time in Pinus genus a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis from immature seeds of
radiata pine. Somatic embryos were obtained from embryogenic line 2162 of Pinus radiata D. Don on EDM basal medium containing 60 μM ABA and 6% sucrose. The explants used for organogenesis experiments were either
freshly collected somatic embryos or somatic embryos germinated for 1 week. Germination medium was half-strength LP medium,
supplemented with 0.2% activated charcoal. Different induction periods and BA concentrations were assayed for shoot induction.
After induction treatments, explants were elongated on the same medium used for germination stage. Rooting medium was quarter-strength
LP medium supplemented with three different auxin treatments: 1.5 mg L−1 1-naphthalene acetic acid (NAA), 1.5 mg L−1 indole-3-butyric acid (IBA) and 1 mg L−1 IBA with 0.5 mg L−1 NAA (MIX). The effect of the photon flux (120 mmol m−2 s−1 and darkness) in the first week of the explants in the rooting media was also tested. This methodology could offer an alternative
to overcome some problems associated with somatic embryogenesis such as the seasonality of embryogenic tissue (ET) initiation
or a low embryo production from the ET, a particularly important issue in the case of genetically transformed ETs. |
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