Transient and stable expression of hepatitis B surface antigen in tomato (Lycopersicon esculentum L.) |
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| Authors: | L Srinivas G B Sunil Kumar T R Ganapathi C J Revathi V A Bapat |
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| Institution: | (1) Nuclear Agriculture and Biotechnology Division, Plant Cell Culture Technology Section, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India;(2) Shantha Biotechnics Limited, Medchal, Hyderabad, 501 401, India |
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| Abstract: | Cotyledonary leaves of tomato cv. Megha were transformed with the hepatitis B virus ‘s’ gene, which encodes surface antigen.
Six plant expression cassettes (pHBS, pHER, pEFEHBS, pEFEHER, pSHER and pEFESHER) were used to assay the possible expression
levels by agroinfiltration. The maximum transient expression level of 489.5 ng/g D.W. was noted in pEFEHER-infiltrated cotyledonary
leaves. Transgenic tomato plants with pEFEHBS and pEFEHER expression cassettes were regenerated and characterized by molecular
analysis. The expression of the antigen in the fruits was confirmed by RT-PCR and ELISA analysis. This is the first report
on the expression of hepatitis B surface antigen in tomato. |
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| Keywords: | Agroinfiltration Edible vaccine ER-retention signal Hepatitis B surface antigen Tomato |
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