Growth retardants stimulate guggulsterone production in the presence of fungal elicitor in fed-batch cultures of <Emphasis Type="Italic">Commiphora wightii</Emphasis> |
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Authors: | Satish Suthar K G Ramawat |
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Institution: | (1) Laboratory of Bio-Molecular Technology, Department of Botany, M. L. Sukhadia University, Udaipur, 313001, India; |
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Abstract: | Guggulsterone, a hypolipidemic natural agent, is produced in resin canals of the plant Commiphora wightii. In this study, the stimulatory effects of growth retardants ALAR (N,N-dimethylaminosuccinamic acid) and CCC (chlormequat chloride)] and fungal elicitor on guggulsterone accumulation in cell cultures
of C. wightii are reported. CCC at 1 mg l−1 enhanced guggulsterone content (~123 μg l−1) when added on the fifth day after inoculation, while ALAR at 2.5 mg l−1 increased guggulsterone content (~116 μg l−1) when added on the tenth day. In a two-stage fed-batch process, combined treatment with fungal elicitor and growth retardant
caused a significant increase (~353 μg l−1) in guggulsterone content in cell cultures after 17 days of growth. This represents an approximately fivefold increase over
the guggulsterone contents in initial cultures of this plant. |
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