Increased tRNA level in yeast cells with mutant translation termination factors eRF1 and eRF3 |
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Authors: | G A Zhouravleva S E Moskalenko S V Chabelskaya M Philippe S G Inge-Vechtomov |
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Institution: | (1) Department of Genetics and Breeding, St. Petersburg University, St. Petersburg, 199034, Russia;(2) Universite de Rennes 1, Rennes, CNRS UMR 6061, IFR 97, 35043, France |
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Abstract: | We have earlier characterized Saccharomyces cerevisiae strains with mutations of essential SUP45 and SUP35, which code for translation termination factors eRF1 and eRF3, respectively. In this work, the sup45 and sup35 nonsense mutants were compared with respect to the levels of eight tRNAs: tRNATyr, tRNAGln, tRNATrp, tRNALeu, tRNAArg (described as potential suppressor tRNAs), tRNAPro, tRNAHis, and tRNAGly. The mutants did not display a selective increase in tRNAs, capable of a noncanonical read-through at stop codons. Most of the mutations increased the level of all tRNAs under study. The mechanisms providing for the viability of the sup45 and sup35 nonsense mutants are discussed. |
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Keywords: | tRNA Saccharomyces cerevisiae translation termination eRF1 eRF3 |
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