Lectins and also bacteria modify the glycosylation of gut surface receptors in the rat

Oral exposure to lectins or the presence or absence of bacteria in the rat small intestine were shown by histological methods using anti-lectin antibodies or digoxigenin-labelled lectins to have major effects on the state of glycosylation of lumenal membranes and cytoplasmic glycoconjugates of epithelial cells. Taken together with the dramatic effects of exposure to lectins on gut function, metabolism and bacterial ecology, this can be used as a basis for new perspectives of biomedical manipulations to improve health. Abbreviations: DIG, digoxigenin-labelled; POD, peroxidase-labelled; Spf, specific pathogen-free; TBS, Saline (0.9% w/v NaCl solution) buffered with 0.05m Tris-glycine, pH 7.8; PAP, antibody-peroxidase-antiperoxidase. For lectins see Table 1.