Structure of the main ganglioside from the brain of Xenopus laevis |
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| Authors: | Rizzo Angela Maria Berra Bruno Rossi Federica Guerra Anna Gornati Rosalba Bernardini Giovanni Taki Takao Kasama Takeshi Mauri Laura Sonnino Sandro |
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| Institution: | (1) Institute of General Physiology and Biochemistry, 20134 Milan, Italy;(2) Department of Structural and Functional Biology, University of Insubria, 21100 Varese, Italy;(3) Molecular Medical Science Institute, Otsuka Pharmaceutical Co. Ltd., Tokushima, Japan;(4) Instrumental Analysis Research Center for Life Science, Tokyo Medical and Dental University, Tokyo, Japan;(5) Center of Excellence on Neurogenerative Diseases, Study Center for the Biochemistry and Biotechnology of Glycolipids, Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, 20090 Segrate, Italy |
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| Abstract: | The main component of the ganglioside1 mixture from the brain of the adult amphibian Xenopus laevis accounts for 35% of the total, as lipid bound sialic acid. This ganglioside has been purified and characterized by thin layer chromatography, partial and exhaustive enzymatic hydrolysis with sialidase, TLC-overlay procedures with anti-Gg4Cer and anti-Neu5Ac 6GalNAc specific monoclonal antibodies and mass spectrometry. All together the results suggest the following structure:Neu5Ac8Neu5Ac3Gal3(Neu5Ac8Neu5Ac6)GalNAc4Gal4Glc1Ceror, IV3--Neu5Ac2,III6--Neu5Ac2-Gg4Cer. |
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| Keywords: | gangliosides structure Xenopus laevis amphibia |
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