The purification of the three trimannosyl-N-acetylchitobiose pentasaccharides from the urine of swainsonine-intoxicated sheep |
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| Authors: | Yves Goussault Christopher D Warren Birgitte Bugge Roger W Jeanloz |
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| Institution: | (1) Laboratory for Carbohydrate Research, Departments of Biological Chemistry and Medicine, Harvard Medical School and Massachusetts General Hospital, 02114 Boston, MA, USA |
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| Abstract: | A simple method for the preparative resolution of three Man3GlcNAc2 isomers called Ia, Ib and II has been designed. It consists mainly of the use of concanavalin A-Sepharose which allowed the total purification of Man3GlcNAc2-Ia, and then of anion-exchange resin in borate buffer-gradient to separate the Ib and II isomers. The purity of each oligosaccharide was checked by two HPLC methods. The use of these oligosaccharides for different analytical and biosynthetic purposes is discussed, and the unexpected resistance of one of the Man3GlcNAc2 alditols to the action of endo- -N-acetylglucosaminidase H is noted. |
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| Keywords: | glycoprotein oligosaccharide purification |
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