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剑尾鱼线粒体细胞色素b基因的序列分析
引用本文:叶星,白俊杰,劳海华,简清,吴淑勤.剑尾鱼线粒体细胞色素b基因的序列分析[J].中国实验动物学报,2002,10(3):131-134.
作者姓名:叶星  白俊杰  劳海华  简清  吴淑勤
作者单位:中国水产科学研究院珠江水产研究所,农业部热带亚热带鱼类选育与养殖重点开放实验室,广州,510380
基金项目:国家公益研究专项资金资助项目—剑尾鱼水生实验动物化研究 (NO :3 40 43 )
摘    要:目的 克隆和测定剑尾鱼 (Xiphophorushelleri)线粒体细胞色素b基因 (cytb)的全序列。方法 提取剑尾鱼肝脏的总DNA。设计合成特异引物进行PCR扩增。扩增产物经琼脂糖电泳检测、纯化后克隆到pGEM Teasyvectorsystem中的T载体上 ,筛选转化子 ,提取质粒 ,酶切鉴定。挑取重组质粒pGEM T xhcytb 11进行序列测定。结果 获得了剑尾鱼线粒体cytb基因的全序列 ,共 114 0bp。结论 用BLAST与GenBank中的线粒体DNA序列进行比较 ,显示剑尾鱼与其他鱼类的cytb基因具有较高的同源性 ;根据剑尾鱼与其他 13种鱼的cytb基因序列同源性所建立的进化树 ,与传统的分类地位基本吻合

关 键 词:剑尾鱼  线粒体  细胞色素b  基因
文章编号:1005-4847(2002)03-0131-04
修稿时间:2001年10月23

Sequence Analysis of Mitochondrial Cytochrome b Gene of Swordtail Fish (Xiphophorus helleri)
YE Xing,BAI Junjie,LAO Haihua,JIAN Qing,WU Shuqin.Sequence Analysis of Mitochondrial Cytochrome b Gene of Swordtail Fish (Xiphophorus helleri)[J].Acta Laboratorium Animalis Scientia Sinica,2002,10(3):131-134.
Authors:YE Xing  BAI Junjie  LAO Haihua  JIAN Qing  WU Shuqin
Abstract:Objective To clone and analyze the whole sequence of mitochondrial cytochrome b (cyt b) gene of swordtail fish ( Xiphophorus helleri ).Methods\ Total DNA from the liver tissue of swordtail fish was extracted and a pair of specific primers were designed and processed for PCR amplification.Analyzed by agarose gel electrophoresis,the amplification products were purified and cloned into T vectors of pGEM\|T easy vector system.With transformants selected and recombinant plasmids extracted,the inserted gene was sequenced.Results\ The whole sequences of cyt b gene of swordtail fish were obtained.Conclusion\ Compared with sequences of cyt b genes in GenBank with BLAST,there were good homologies among swordtail fish and other fishes.The evolution tree built from the homology of cyt b genes of swordtail fish and other 13 kinds of fishes was consistent with the traditional taxonomic results.
Keywords:Xiphophorus helleri  Mitochondrion  Cytochrome b  Gene
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