GnRH-A免疫与母兔生殖激素浓度的变化

目的探讨促性腺激素释放激素类似物（GnRH-A）对动物生殖功能调节的效果和作用机制。方法 24只日本大耳白兔分为四组,分别在实验Ⅰ组（EG-I）、实验Ⅱ组（EG-II）和实验III（EG-III）组兔的颈背侧注射1.0 mL（100、100和50μg/mL）GnRH-A抗原,实验II组和实验III组于第3周以原剂量加强注射一次,用ELISA法测定血清GnRH抗体效价、促卵泡刺激素（FSH）和促黄体生成素（LH）含量。结果注射GnRH-A后10 d实验组兔均出现GnRH抗体,而对照组未检测到;EG-I在第30天达到高峰,而EG-II和EG-III于40～50 d至峰值,但在实验结束时（70 d）实验组均高于对照组,40～70 d时EG-II显著高于EG-I和EG-III。30～50 d时EG-II的LH明显高于EG-I和EG-III及对照组。EG-II和EG-III的FSH浓度在40 d达到峰值,但EG-II高于EG-I、对照组及EG-III,EG-I和对照组无显著差异。结论兔体内注射GnRH-A可以明显提高GnRH抗体效价,增强LH和FSH的合成与分泌,加强注射效果更明显,且与注射剂量相关,持续时间为40 d左右。

GnRH-A Immunization and the Changes of Reproductive Hormone Concentration in Female Rabbits

Objective The objective of this study was to investigate the efficacy and mechanisms of reproductive function immunological regulation by injecting gonadotropin releasing hormone analogue（GnRH-A） in animals.Methods 24 female rabbits were divided randomly into four groups,namely experimental group 1（EG-I）,experimental group 2（EG-II）,experimental group 3（EG-III） and control group（CG）.The experimental groups were injected with GnRH-A antigen 1.0 mL at different doses（100 μg /mL,100 μg /mL and 50 μg /mL）,respectively,and the rabbits of EG-II and EG-III groups were re-injected at the original doses at the third week.Serum GnRH antibody titer,follicle-stimulating hormone（FSH） and luteinizing hormone（LH） concentrations were determined by ELISA.Results Serum GnRH antibody titers were detected in three experimental groups after 10 d of injecting GnRH-A,but not in the control group.The antibody titers of EG-I,EG-Ⅱ and EG-Ⅲ reached a peak level on d30 and d40 ～ d50,respectively.The antibody titers of EG-II were higher than that of EG-I and EG-III（P 0.01）during the period from 40 days to 70 days.LH concentration of EG-II exceeded other groups at 30 d-50 d.FSH concentrations of EG-II and EG-III reached the peak on 40 d,EG-II FSH was higher than other groups（P 0.05）.There was no significant difference between EG-Iand CG.Conclusion Injecting GnRH-A to rabbits may apparently improve GnRH antibody titer,enhance LH and FSH synthesis and secretion.Strengthening injection is more effective,lasting for about 40 days,and is related with the injecting dosage.