硝酸甘油消化道吸收后在动物体内的代谢研究

目的　观察硝酸甘油 (glyceryltrinitrate ,GTN)经消化道吸收后的分解代谢机理。方法　以实验兔为动物模型 ,应用电生理监测仪动态检测血压 ,血气分析仪检测高铁血红蛋白 (MetHb) ,全自动生化分析仪检测丙氨酸氨基转移酶 (ALT) ,比色法检测硝酸盐、亚硝酸盐、还原型谷胱甘肽 (GSH)。结果　GTN经消化道吸收后代谢产生亚硝酸盐 ,实验动物血压、红细胞GSH降低 ,MetHb、ALT未见明显变化。结论　机体内GTN分解代谢过程中可消耗还原性巯基和产生亚硝酸盐 ,对血红蛋白和肝脏功能无明显影响。

Studies of Metabolic Glyceryl Trinitrate Absorbed through Alimentary Canal

Objective To investigate the metabolic derivatives of the glyceryl trinitrate (GTN). Methods Rabbits were used in this work. Blood pressures were measured by dynamic analyzer, methemoglobin (MetHb) by blood gas analyzer and alanine transferase (ALT) by auto-biochemist analyzer. Nitrate, nitrite and reduced glutathione (GSH) were measured with colorimetry. Results Blood pressures and GSHs in red blood cells showed significant decrease, nitrite showed significant increase, MetHb and ALT showed no remarkable difference before and after GTN administration. Conclusion Metabolic GTN exhausts thiols and yields nitrite, and it has no effects on hemoglobin and liver.