| 磁共振成像对扩张型心肌病转基因模型小鼠左右心室对比分析 |
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| 引用本文： | 朱皓,吕丹,高凯,张连峰.磁共振成像对扩张型心肌病转基因模型小鼠左右心室对比分析[J].中国实验动物学报,2013,21(2):28-33,I0004. |
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| 作者姓名： | 朱皓 吕丹 高凯 张连峰 |
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| 作者单位： | 朱皓 (中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,北京100021)；吕丹 (中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,北京100021)；高凯 (中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,北京100021)； 张连峰 (中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,北京100021国家中医药管理局人类疾病动物模型三级实验室,北京100021)； |
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| 基金项目： | 【基金项目]国家“重大新药创制”科技重大专项课题“啮齿类研发平台创新药物研究开发技术平台建设”（2011ZX09307-302）和国家科技支撑计划课题“神经和代谢疾病基因工程模型的建立”（2012BA139802）. |
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| 摘 要： | 目的对cTnTR141W扩张型心肌病转基因模型小鼠左、右心室进行对比分析,研究cTnTR141W转基因小鼠作为右心室心肌病的动物模型的可行性。方法利用7.0 T高场强磁共振成像(MRI)技术,定量分析了2、4、6和8月龄对照组及cTnTR141W转基因模型小鼠左、右心室的舒张末容积(EDV)、收缩末容积(ESV)和射血分数(EF)的变化情况,同时对6月龄对照组cTnTR141W转基因模型小鼠心肌组织进行组织学分析。结果转基因阴性对照小鼠相比,cTnTR141W转基因小鼠左、右心室的容积在2月龄时已有增大趋势,而射血分数有减小趋势。右心室射血分数减小出现最早也最显著(P<0.05)。随年龄增加,cTnTR141W转基因小鼠与转基因阴性对照小鼠相比,右心室的结构和功能的病理生理变化与左心室同时趋于严重。该小鼠左、右心室在4月龄后表现典型的扩张型心肌病表型。结论 cTnTR141W转基因模型小鼠左心室和右心室的扩张性心肌病表型同时出现,该小鼠可作为右室性心肌病等右心室功能下降相关疾病研究的动物模型。
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| 关 键 词： | 扩张型心肌病 转基因小鼠模型 磁共振成像 射血分数 |
Comparative analysis of left and right ventricular dilated cardiomyopathy in transgenic mice by magnetic resonance imaging |
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| ZHU Hao,LU Dan,GAO Kai,ZHANG Lian-feng.Comparative analysis of left and right ventricular dilated cardiomyopathy in transgenic mice by magnetic resonance imaging[J].Acta Laboratorium Animalis Scientia Sinica,2013,21(2):28-33,I0004. |
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| Authors: | ZHU Hao LU Dan GAO Kai ZHANG Lian-feng |
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| Institution: | 1. Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Beijing 100021, China; 2. Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing 100021 ) |
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| Abstract: | Objective To compare the left and right ventricular function of cTnTR141W transgenic mice with dilated cardiomyopathy, and to assess whether cTnTR141W transgenie mice can he used as an animal model of right ventricular car- diomyopathy. Methods The structural and functional changes of left and right ventricular myocardium in non-transgenic mice （NTG） and cTnTR141Wtransgenic mice at 2, 4, 6 and 8 months of age were assessed and analyzed by 7.0 T high-field magnetic resonance imaging technologY. Changes of the left and right ventricular end-diastolic volume （EDV） , end-systolic volume （ESV） , and ejection fraction （EF） were quantitatively analyzed with ages. Pathologic changes of myocardial tissue from NTG and cTnTR141W transgenic mice at 6 month of age were. analyzed. Results Compared with the NTG mice, the left and right ventricular volume of cTnTR141W transgenic mice showed an increasing tendency, and the ejection fraction showed a decreasing trend, The right ventricular ejection fraction showed earliest significant difference between NTG and cTnTz141wtransgenic mice （ P 〈 0. 05）. Compared with the NTG mice, the structural and pathophysiological changes of the right and left ventricles of cTnTR141W transgenic mice simuhaneouisly became serious with aging. Typical dilated cardiomyopathy phe- notype occurred in the right and left ventricles after 4 months of age. Conclusions The dilated cardiomyopathy phenotype of the left and right ventricles in cTnTsl41w transgenic mice occurred simultaneously. These mice can be used as an animal model for research on right ventricular cardiomyopathy or other diseases related with decreased function of the right ventricle, |
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| Keywords: | Dilated cardiomyopathy Transgenic mice Magnetic resonance imaging Ejection fraction |
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