| 大鼠肠内容物细菌基因组DNA提取方法的比较 |
| |
| 引用本文： | 戴方伟,宋晓明,周莎桑,柯贤福,李长龙,应华忠,萨晓婴.大鼠肠内容物细菌基因组DNA提取方法的比较[J].中国实验动物学报,2011,19(3):246-249. |
| |
| 作者姓名： | 戴方伟 宋晓明 周莎桑 柯贤福 李长龙 应华忠 萨晓婴 |
| |
| 作者单位： | 浙江省医学科学院,浙江省实验动物与安全性研究重点实验室,杭州310013 |
| |
| 基金项目： | 浙江省实验动物科技计划项目，浙江省卫生厅省部共建项目 |
| |
| 摘 要： | 目的比较两种肠内容物前处理和两种提取方法对清洁级SD大鼠肠内容物细菌基因组DNA提取效率。方法分别选用PBS多次离心漂洗、液氮破细胞两种前处理方法和酚/氯仿抽提、试剂盒过柱法两种提取方法进行组合分析,对4份肠内容物和16份含金黄色葡萄球菌肠内容物进行随机提取。结果大鼠肠内容物细菌基因组DNA含量和纯度测定结果显示,与PBS反复离心相比,液氮研磨前处理能显著提高大鼠肠内容物基因组DNA。荧光定量PCR表明,液氮研磨前处理较PBS反复离心能更好地收集细菌基因组DNA,其Ct值最低。结论研究结果表明,采用液氮研磨试剂盒法在大鼠肠内容物DNA提取中是较为优良的方法,该方法为建立实验动物中微生物的定量PCR检测方法打下了基础。
|
| 关 键 词： | 实验动物 肠内容物 细菌 DNA提取方法 |
Comparison of bacterial genomic DNA extraction methods from Sprague-Dawley rat fecal samples |
| |
| DAI Fang-wei,SONG Xiao-ming,ZHOU Sha-sang,KE Xian-fu,LI Chang-long,YING Hua-zhong,SA Xiao-ying.Comparison of bacterial genomic DNA extraction methods from Sprague-Dawley rat fecal samples[J].Acta Laboratorium Animalis Scientia Sinica,2011,19(3):246-249. |
| |
| Authors: | DAI Fang-wei SONG Xiao-ming ZHOU Sha-sang KE Xian-fu LI Chang-long YING Hua-zhong SA Xiao-ying |
| |
| Institution: | DAI Fang-wei,SONG Xiao-ming,ZHOU Sha-sang,KE Xian-fu,LI Chang-long,YING Hua-zhong,SA Xiao-ying(Zhejiang Academy of Medical Sciences,Zhejiang Provincial Laboratory of Experimental Animal and Safety Evaluation Research,Hangzhou 310013) |
| |
| Abstract: | Objective To compare four methods of bacterial genomic DNA extraction from Sprague-Dawley rat fecal samples.Methods Sixteen fecal samples with 106 cfu S.aureus and 4 fecal samples without S.aureus were equally divided into four groups.Bacterial genomic DNA were extracted by following methods:(1) Several wash by PBS plus phenol/chloroform purification;(2) Liquid nitrogen digestion plus column purification;(3) PBS wash plus column purification,and(4) Liquid nitrogen digestion followed by phenol/chloroform pur... |
| |
| Keywords: | Laboratory Animal Fecal Sample Bacteria DNA extraction methods |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
