氟骨症不同动物模型的建立与评价

目的 为选择合适的实验动物并建立氟骨症动物模型,研究不同动物对氟化物致骨骼损伤的敏感性.方法 分别选取离乳新西兰兔、Hartley豚鼠、Wistar大鼠、KM小鼠各16只,每种动物随机分为两组,每组8只,即对照组(C):饲喂标准饲料,饮用去离子水;模型组(M):饲喂标准饲料,饮用150 mg/L氟化钠(NaF)去离子水.在饲喂60 d后取股骨制作组织切片观察组织形态变化,并测定股骨骨氟、骨钙、骨磷的含量及血清碱性磷酸酶(ALP)活性.结果 与对照组相比,各种实验动物模型组生长发育受到显著的抑制,股骨形态破坏明显,骨矿化显著减少,骨氟含量显著升高,血清ALP活性显著增强,但股骨钙和磷含量差异无显著性(P＞0.05).结论 高氟可不同程度的损害豚鼠、家兔、小鼠、大鼠的生长发育及骨骼系统,导致产生氟骨症,但综合考察,以豚鼠、家兔较为敏感,其次为小鼠、大鼠.

Establishment and evaluation of four animal models of skeletal fluorosis

Objective To select and establish suitable experimental animal models of skeletal fluorosis and analyze their sensitivity to fluoride-induced bone damages.Methods New Zealand rabbits,Hartley white guinea-pigs,Wistar rats,and Kunming mice(16 each) were included in this study.Each kind of the animals were divided randomly into two groups(each group 8): control group(group C) fed with standard food and distilled water,and model group(group M) fed with normal food and 150 mg/L NaF distilled water).At 60 days af...