老龄ICR小鼠对SARS-CoV的易感性

目的为探讨SARS的发病机制并提供易感的SARS动物模型。方法利用RT-PCR和病毒分离后免疫荧光技术检测成龄鼠和老龄鼠接种SARS-CoV后肺组织内病毒复制情况,同时观察两组动物的肺脏和肺外组织器官的病理变化,对肺组织进行免疫组化分析,观察SARS-CoV在肺内复制的主要部位。结果老龄鼠的感染率明显高于成龄鼠;老龄鼠肺脏出现更为严重的弥漫性肺泡损伤,其中两只老龄鼠的肺外器官出现了变性、灶状坏死以及血管广泛的扩张充血等全身中毒性变化;肺脏内病毒抗原主要存在于肺泡上皮细胞和间质的血管内皮细胞。结论老龄ICR小鼠对SARS-CoV的易感性明显高于成龄鼠,有可能作为研究SARS发病机制以及药物评价的动物模型。

Susceptibility of Aged ICR Mice to SARS-CoV

Objective To establish an animal model sensitive to SARS-CoV and study the pathogenesis of the disease.Methods Using RT-PCR and immunofluorescence assay(IFA) after virus islation and inoculation, SARS-CoV was examined in the lungs of young and aged ICR mice challenged by SARS-CoV.Pathological changes in the lungs and other organs of the two groups were examinined.The replication sites of virus in the lungs were analyzed by immunohistochemistry staining.Results Aged mice were more susceptible to SARS-CoV than young mice do.More severe diffuse alveolar damages were observed in aged mice.In addition,damages were observed in other organs of two aged mice such as degeneration,focal necrosis and vein dilation and congestion in liver,kidney and brain.Virus antigen was detected in alveolar epithelial and vascular endothelial cells.Conclusion Aged ICR mice may be used as a susceptible animal model for SARS-CoV in studies of mechanisms of SARS and evaluation of the effect of antiviral drugs and vaccines.