山羊体外受精的研究

通过在山羊卵母细胞体外成熟培养液中添加不同的血清和不同浓度的卵泡液 ,在体外成熟培养液中培养不同的时间 ,以及采用不同的精子获能方法来摸索效率较高的体外受精方法体系。在山羊卵母细胞体外成熟培养液中添加FCS、EGS及不同浓度的卵泡液 ,成熟培养时间分别为 16、2 0、2 4和 2 7h ,山羊新鲜精液用钙离子载体法和肝素法进行获能处理后用于体外受精 ,比较其体外成熟率和体外受精率。添加FCS、EGS和 2 0 %卵泡液组的成熟率无显著差异 ,显著高于添加 10 %和 3 0 %卵泡液组的成熟率 ;但添加FCS和EGS组的受精率显著高于添加10 %、2 0 %、3 0 %卵泡液组的受精率。培养 2 4h组和 2 7h组的成熟率显著高于另外两组 ,而培养 2 7h组的受精率显著高于其余各组。用钙离子载体法处理的山羊精子的顶体反应率和体外受精率显著高于肝素法。EGS可以代替FCS添加于成熟培养液中 ,对COCs的成熟率和受精率没有明显的影响 ,但卵泡液不能完全代替FCS的作用。培养时间为 2 7h ,精子用钙离子载体法处理获能后能得到较高的体外受精率

Study on Goat in vitro Fertilization

ObjectiveTo analyze the maturation rate and fertilization ratio of goat oocytes cultured in different mediums for different time or spermatozoa were incubated in two dif ferent mediums.Mothods\ The COCs were cultured in mediums supplemen ted with FCS, EGS or different concentration of follicular fluid and were cultur ed in the same culture medium for 16hrs,20hrs,24hrs or 27hrs,spermatozoa were ca pacitated in medium supplemented with heparin or IA 23187.The maturation rate a nd the fertilization ratio were statistically compared. Resullts\ Oocytes cultured in medium supplemented with FCS or EGS for 27hrs had highe r matured rate and fertilization ratio.Sperm capacitated in medium supplemented with IA 23187 had higer capacitation rate and fertilization ratio.Co nclusion\ COCs cultured in medium supplemented with FCS or EGS for 27hrs and sperm capacitated in medium supplemented with IA 23187 is an efficient IVF s ystem.