利用裸鼠建立人泌尿生殖系统肿瘤细胞系

目的建立人泌尿系肿瘤无限细胞系,为泌尿系肿瘤研究提供实验模型.方法无菌取下肿瘤标本后,将标本剪成大小约1.0mm3的组织块,在裸鼠右后肢皮下包埋,当皮下肿瘤块发生明显增殖并长到一定程度后,再行裸鼠体内传代两次,最后取下组织块进行原代培养.培养细胞传代超过20代后按建系标准2]进行检测.结果共取40例标本,裸鼠体内传代F1代成功6例,F3代成功3例,该3例标本行原代培养后建成3个无限细胞系人肾透明细胞癌RCC-9863,人膀胱癌BC-6,人前列腺癌PC-98106,全部细胞传代1年以上,生长稳定,传代周期固定,其形态结构,分化程度与原发瘤保持一致,染色体形态仍为人类核型.结论裸鼠肿瘤皮下种植法是泌尿系肿瘤建系的一个较好方法.

Establishment of Urogenital Infinite Tumor Cell Lines by Nude Mice Tumor Xenotransplantation

Objective\ To establish urogenital carcinoma cell lines and provide a tumor model for further research.Methods\ The tumor tissues were cut into several fragments sized 1.0mm3 then implanted into one nude mouse subcutenousely.If the tumor tissues in the nude mouse appeared to grow and became very large,it then was passaged in nude mice for another two times.The tumor tissues in the third mouse was cut and cultured in vitro primarily.When the cells were cultured more than 20 passages,it was certified according to the references.Results\ 40 specimens were implanted.6 tumors grew in F 1 nude mouse,and 3 in F 3. The 3 tumor tissues were incised and cultured in vitro.Finally 3 infinite tumor cell lines were established:renal carcinoma cell line RCC-9863;bladder cancer cell line BC-6;prostate cancer cell line PC-98106.They are all cultured for more than 1 years and grow steadly.Also they have the human's morphological structure and inherent characters.Conclusion\ Nude mouse tumor implantation model is considered to be a good method to establish infinite urogenital carcinoma cell lines.