人肝癌原位移植转移模型特性实验研究

目的　建立人肝癌细胞系HCC-9724(简称H)淋巴结转移模型，研究肿瘤转移机理。方法　采用裸鼠肝脏原位移植法，接种肿瘤细胞，取其淋巴结转移灶反复肝内接种，连续传三代后，观察其转移特性，采用SABC法测定淋巴结中nm23和Ⅳ型胶原酶表达。结果　裸鼠原位接种50d，肝内长出约1.7cm×6.0cm大小的肿瘤，呈分叶状，质地较软，周围血供丰富，瘤组织与邻近脏器粘连，有明显的浸润和转移，经裸鼠三次筛选后肿瘤潜伏期短(15d)，瘤体大，形成广泛的肠系膜淋巴结转移，淋巴结中Ⅳ型胶原酶表达呈强阳性；而nm23呈弱阳性。结论　采用裸鼠肝原位移植法，反复筛选，获得了人肝癌淋巴结高转移模型。

The Study of Human Hepatic Carcinoma Metastasis pro perties by Orthotopic Transplantation in Nude Mouse

Objective To establish lymph node metastatic animal model ofhuman hepatic carcinoma for the study of m etastasis mechanism.Methods HCC-9724 was in oculated in nude mouse liver.The metastatic lymph node focuses were reinoculate d repeatedly to nude mouse liver，3 times later，the metastasis characters were determined by SABC of the expression of nm23 and Ⅵ collagenase in lymph nodes. Results 50 days after orthotopic transplant ation，tumor mass sized 1.7 cm×0.6 cm had grown，lobulate，soft，more blood v essels，with apparent invasion and metastasis.After 3 passages in nude mouse，compared with HCC-9724 the latent period of tumor became short.It had la rgesize，wide metastasis to mesent eric lymph node (P＜0.05)，expression of possitive Ⅳ collagenase and negative nm23 gene.Conclusion The high m etastatic animal model of human hepatic carcinoma was successfully established b y orthotopic transplantation with continuous passage generation.