哺乳期BALB/c小鼠胆道梗阻模型的探索

目的探讨新生BALB/c小鼠胆道梗阻模型的建立,并与报告的新生BALB/c小鼠感染猕猴轮状病毒(RRV)模型小鼠生存曲线进行比较。方法将出生后5～7 d的BALB/c小鼠随机分为实验组和对照组,实验组进行胆总管结扎,然后关腹。对照组打开腹部后关腹不结扎胆总管。实验完成后每天观察小鼠的体重变化、无毛区皮肤颜色变化、小鼠存活天数以及在术后第5、10天时分别取小鼠肝脏做病理及免疫组化。结果小鼠在结扎后随着时间的延长,小鼠的体重及肝体比、无毛区皮肤颜色、存活天数、肝脏病理等都存在一定变化。小鼠体重增长逐渐缓慢,术后第2天就会出现无毛区的皮肤变黄,在尿道口会有淡黄色的液体并随后出现陶土样便。在术后第5天及第10天时取肝脏做肝体比有统计学差异(P≤0.05),小鼠在术后第10天左右会出现死亡高峰。结论新生BALB/c小鼠胆总管结扎模型是研究胆道梗阻的可靠动物实验,其生存曲线与报告的猕猴轮状病毒致胆道闭锁大体类似。

Exploration of establishment of a newborn BALB/c mouse model of biliary obstruction

Objective The aim of this preliminary study was to establish a newborn BALB/c mouse model of biliary obstruction, and to compare with reported neonatal BALB/c mice with rhesus monkey rotavirus （RRV） infection. Methods Twenty-four postnatal 5 to 7 days old BALB/c mice were randomly divided into experimental and control groups. In the experimental group the common bile duct was ligated, and then the abdomen was closed. In the control group the abdo- men was opened without ligation of the common bile duct. After the completion of the experiment the body weight of the mice, hairless skin color changes, and the mouse survival days were observed daily. The liver pathology and immunohisto- chemistry were examined at 5 and 10 days after ligation. Results In the mice after ligation, the body weight and liver/ body weight ratio, skin color of the hairless area, the number of days of survival, and liver pathology showed some chan- ges. The body weight gain gradually slowed down and after two days the skin of hairless area turned yellow, yellow liquid at the urethra opening and then clay stools occurred. There were statistically significant differences （ P 〈 0. 05 ） between the liver coefficients at postoperative days 5 and 10. The death peak occurred around 10 days after operation. Conclusions The established newborn BALB/c mouse model of common bile duct ligation is a reliable model for studies of biliary ob- struction. Its survival curve is broadly similar to that of reported rhesus rotavirus （RRV） -induced biliary atresia.