| 利用RAPD技术分析实验用比格犬的遗传背景 |
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| 引用本文： | 黄朝峰,仲乃琴,谭萍萍,段子渊,陈伟平,刘运忠,罗甜仪.利用RAPD技术分析实验用比格犬的遗传背景[J].中国实验动物学报,2002,10(2):65-68. |
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| 作者姓名： | 黄朝峰 仲乃琴 谭萍萍 段子渊 陈伟平 刘运忠 罗甜仪 |
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| 作者单位： | 1. 中国科学院遗传与发育生物学研究所,北京,100101
2. 广东省广州市医药工业研究所,广州,510240 |
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| 摘 要： | 目的 运用RAPD技术对实验用比格犬 (Beagle)进行遗传背景分析。方法 筛选的 16个RAPD引物对 4 0个样品的分析 ,共得到 93个扩增条带。结果 所有样品的相似系数 80 4 %到 10 0 %间变动 ,平均相似系数为93 2 8% ,聚类分析得到了这些个体的同源树资料。结论 本遗传背景资料可以为以后的比格犬繁育生产和生物医药学的研究应用提供技术指导
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| 关 键 词： | 随机扩增多态性DNA 比格犬 遗传背景分析 |
| 文章编号： | 1005-4847(2002)02-0065-04 |
| 修稿时间： | 2002年3月12日 |
Analysis for Genetic Background of Laboratory Beagle Dog with Random Amplified Polymorphic DNA(RAPD) |
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| HUANG Zhaofeng,ZHONG Naiqing,TAN Pingping,DUAN Ziyuan ,CHEN Weiping,LIU Yunzhong,LUO Tianyi.Analysis for Genetic Background of Laboratory Beagle Dog with Random Amplified Polymorphic DNA(RAPD)[J].Acta Laboratorium Animalis Scientia Sinica,2002,10(2):65-68. |
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| Authors: | HUANG Zhaofeng ZHONG Naiqing TAN Pingping DUAN Ziyuan CHEN Weiping LIU Yunzhong LUO Tianyi |
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| Institution: | HUANG Zhaofeng,ZHONG Naiqing,TAN Pingping,DUAN Ziyuan *,CHEN Weiping,LIU Yunzhong,LUO Tianyi |
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| Abstract: | Objective To analyse Random Amplified Polymorphic DNA(RAPD) for laboratory Beagle dogs so as to know their genetic background.Method\ 16 RAPD primers were selected and used to amplify 40 samples.Results\ In this study,93 bands were gained in agarose gel.The homology coefficient of all samples ranged from 80.4% to 100%,average at 93.28%.Assisted by DNAman software,the homology trees were produced by cluster analysis with all individuals and group samples.Conclusion\ Those genetic background materials can provide some technologic direction for Beagle dog breeding and applying in bio-medicine research. |
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| Keywords: | Random Amplified Polymorphic DNA(RAPD) Beagle dog genetic background analysis |
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